The hydrogenase cofactor H-cluster consists of a [4Fe-4S] cluster and a [2Fe] cluster.

Recently, researchers at the University of Bochum, Ruhr (RUB) in Germany used semi-synthetic methods to prepare biologically active hydrogenases using inert iron complexes and protein biosynthetic precursors.

Currently it is very difficult to extract hydrogenase from living cells, so it will take a long time for industrial applications in this area. The RUB study has achieved decisive results in bio-based hydrogen production. Related results were published in the journal Nature-Chemical Biology.

Hydrogenases play an important role in maintaining energy balance in many unicellular organisms. For humans, they can help produce clean energy carriers - hydrogen. Therefore, biologists and chemists have worked hard for many years to make these enzymes and their chemical synthesis suitable for industrial applications, such as new fuel cell materials that are economical and environmentally friendly.

Hydrogen is the ideal fuel for fuel cells. It is not only of high purity, but also can be directly used for batteries in fuel cell vehicles. It does not require complicated ancillary equipment and devices such as reformers and purifiers. The hydrogen-fueled fuel cell engine system is relatively simple. The fuel cell starts quickly, has stable performance, and responds quickly to load changes. It is basically "zero pollution" and relatively low cost.

The team found that the catalytic activity known as iron-iron hydrogenase is based primarily on a complex structure of active centers, including iron, carbon monoxide and quinone. In order to skip the tedious and inefficient hydrogenase production process, chemists have recreated catalytically active enzyme components. Although successfully constructed, this chemical imitation produces only a small amount of hydrogen. Therefore, the research team proposed an optimized method for extracting hydrogenase in living organisms, which has been published in the June 2013 issue of Nature.

Hydrogenase has a broad application prospect, but it is still very difficult to industrialize it. Under ideal conditions, a hydrogenase can produce 9,000 hydrogen molecules per second. Nature has created a catalyst that is extremely active in the absence of any precious metals.

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